3D Ex vivo tissue platforms to investigate the early phases of influenza a virus- and SARS-CoV-2-induced respiratory diseasesArticle Published on 2022-12-012022-11-15 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 신약개발, 진단, 치료제, [키워드] animal model animal models Antiviral Cell Cell culture comparable consequence Drug repurposing drug target early phase Ex vivo ex vivo infection Ex vivo infection model Health Host human lung IAV in vitro Infection Inflammatory cytokine Influenza influenza A virus Influenza virus Innate immunity innate immunity. interferon interferon response Intervention introduced lung tissue morbidity and mortality murine outbreak pathophysiological pharmacological platform Respiratory disease respiratory viruses responses SARS-CoV-2 therapy tissue Treatment viral infection viral life cycle viral replication virus virus while [DOI] 10.1080/22221751.2022.2117101 PMC 바로가기
SARS-CoV-2 Diverges from Other Betacoronaviruses in Only Partially Activating the IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in Human Lung-Derived CellsArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] activate Activation activities activity acute respiratory syndrome betacoronaviruses Cell cellular change coronavirus coronavirus country Critical death downstream Endoplasmic reticulum enzyme ER stress examined expression of gene functions Gene Expression HCoV-OC43 Host host cell host cells host immune system Human human cell human cells imperative individual induce infect Infection inhibit interact IRE1α pathway less limit MERS-CoV MHV Middle East molecular mechanism mRNA murine OC43 Other other coronavirus pathway Prevent processed Replication required Respiratory Coronavirus respiratory syndrome coronavirus Reticulum RNase SARS-CoV SARS-CoV-2 SARS-COV-2 infection severe respiratory disease signaling pathway similarity splice Spread Stress tested the interferon transcription factor Trigger unfolded protein response Unfolded protein response. UPR Vaccine viral replication virus XBP1 [DOI] 10.1128/mbio.02415-22 PMC 바로가기
Essential role of TMPRSS2 in SARS-CoV-2 infection in murine airwaysArticle Published on 2022-10-152022-11-16 Journal: Nature Communications [Category] COVID19(2023년), SARS, 변종, [키워드] airway airways Analysis Beta calu-3 cells cathepsin-dependent Cell cleavage cultured cells Essential furin Gamma host proteases in vivo infect Infection isolate mice mouse-adapted SARS-CoV-2 multiple pathway murine omicron pathway recent reduced SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variants shown significantly the spike protein TMPRSS2 variant [DOI] 10.1038/s41467-022-33911-8 PMC 바로가기
Toxicity and virucidal activity of a neon-driven micro plasma jet on eukaryotic cells and a coronavirusArticle Published on 2022-10-012022-11-15 Journal: Free radical biology & medicine [Category] COVID19(2023년), SARS, 치료제, [키워드] abrogated airway antioxidant antiviral activity chronic wound clinics Cold Cold physical plasma complex Contact coronavirus cytotoxic effect cytotoxicity Direct Effect Effects eukaryotic cell fibroblast Gas plasma technology Hepatitis human cell line human cells human coronaviruses identify inactivation increasingly infections mechanism metabolic activity MHV murine murine hepatitis virus number of infected oxygen pandemic Patient physical plasma plethora reactive oxygen reactive oxygen species reduced responsible ROS ROS production Safe substrate the SARS-CoV-2 therapeutic purposes tissue Toxicity treat Treatment unique viability virucidal virus inactivation Virus inactivation. [DOI] 10.1016/j.freeradbiomed.2022.08.026 PMC 바로가기
Prophylactic treatment of Glycyrrhiza glabra mitigates COVID-19 pathology through inhibition of pro-inflammatory cytokines in the hamster model and NETosisArticle Published on 2022-09-272022-11-16 Journal: Frontiers in Immunology [Category] SARS, 변종, [키워드] accompanied acute respiratory distress addition Comorbidity coronavirus disease COVID-19 COVID-19 pathology cytokine production decrease dysregulated immune response effective Efficacy Evidence functions glycyrrhiza Glycyrrhiza glabra Hamster model hamsters hypercoagulable IL-17A IL-4 immunocompromised individuals immunomodulator immunomodulatory properties Inflammatory cytokine ingredient Lung pathology lung viral load mice mice and human neutrophil functions. mitigate Mortality mRNA expression murine NET NETosis neutrophil polymorphonuclear neutrophil pro-inflammatory cytokine Protective provide Pulmonary pathology reduced reducing robust ROS SARS-CoV-2 SARS-COV-2 infection skewed suppress syndrome Th17 Th2 Th2/Th17 differentiation Treatment vaccination Vaccine variant [DOI] 10.3389/fimmu.2022.945583 PMC 바로가기
Bioinformatics and systems-biology analysis to determine the effects of Coronavirus disease 2019 on patients with allergic asthmaArticle Published on 2022-09-232022-11-15 Journal: Frontiers in Immunology [Category] 바이오마커, 유전자 메커니즘, [키워드] addition allergic Allergic asthma Analysis approach association Asthma asthmatic bioinformatics Biomarker can be used candidate drug candidate drugs Comorbidity conducted coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 progression COVID-19 symptom Critical dataset datasets DEGs determine differentially expressed gene disease disease biomarker drug Effect elevated Evidence expression level Gene ontology Genes health systems hub gene identify IL-13 IL-5 Increasing Interaction interactions lung mechanism molecular molecular mechanism MUC5AC murine murine model pandemic pathway Pathway analysis Patient patients with COVID-19 phenotype potential molecular risk factor severity shared pathways statistical Systems biology systems-biology. Transcription [DOI] 10.3389/fimmu.2022.988479 PMC 바로가기
Identification and characterization of a novel cell binding and cross-reactive region on spike protein of SARS-CoV-2Article Published on 2022-09-192022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 진단, [키워드] addition amino acid sequence Antisera Beas-2B cells binding Cell characterized conserved COVID-19 Cross reactivity cross-reactive develop effective vaccine Escherichia coli evade full-length Host host cell identification IgG immune imperative in viral Infection inhibited involved murine Mutation mutations neutralizing monoclonal antibody observation Prevent produced pseudovirus purified Recombinant protein S protein S-protein SARS-CoV SARS-CoV-2 Serologically Spike protein virus virus variant [DOI] 10.1038/s41598-022-19886-y PMC 바로가기
A novel platform for attenuating immune hyperactivity using EXO-CD24 in COVID-19 and beyondArticle Published on 2022-09-072022-10-06 Journal: EMBO Molecular Medicine [Category] 진단, [키워드] adverse event anti-inflammatory drug CD24 cells characterized Combination COVID-19 COVID-19 patient cytokine Cytokine storm develop displaying doses Efficacy examined EXO-CD24 Exosome Exosomes exosomes. hyper-inflammatory response hyperactivity immune in vitro in vivo Inflammatory marker inhibit interfere life-threatening lung murine NF-kB pathway PAMPs pathogen-associated molecular pattern Patient platform produced proportion purified randomized study recruited reduced required severity suppress systemic disease therapeutic Treatment viral clearance [DOI] 10.15252/emmm.202215997 PMC 바로가기 [Article Type] Article
Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerabilityArticle Published on 2022-09-012022-11-15 Journal: Structure (London, England : 1993) [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acute respiratory syndrome analysis antibodies antibody beta-coronavirus beta-coronavirus beta-coronaviruses Broadly neutralizing antibody conformations conserved coronavirus COVID-19 crystal structure elicit hydrophobic residue immunized indicated inhibiting Middle East mRNA murine neutralization neutralize neutralized Neutralizing recognizing S2 subunit S2-directed antibody Sarbecoviruses SARS-CoV SARS-CoV-2 SARS-CoV-2 variants spike syndrome the antibody the SARS-CoV-2 Vaccine design Vaccine design. [DOI] 10.1016/j.str.2022.06.004 PMC 바로가기
Comparative Assessment of Filtration- and Precipitation-Based Methods for the Concentration of SARS-CoV-2 and Other Viruses from WastewaterArticle Published on 2022-08-312022-11-15 Journal: Microbiology Spectrum [Category] SARS, 치료기술, 치료제, [키워드] Adenovirus affected Ammonium Analysis approaches assessment centrifugation Characteristics Community Comparative Concentration COVID-19 COVID-19 pandemic develop Enteric viruses Environmental virology Epidemiology evaluated experiments fecal FIVE Genome glycol human respiratory viruses in viral Influenza information Laboratory Mild murine National Other pathogen detection pathogenic virus physico-chemical property public health public health surveillance quantification rapid expansion SARS-CoV-2 SARS-COV-2 infection selected sewage concentration. subsequent Surveillance tested virus virus virus detection virus particle virus titer viruses Volume wastewater [DOI] 10.1128/spectrum.01102-22 PMC 바로가기